cDNA libraries are being created from zebrafish embryonic
cells expressing LMO-2 promoter driving green fluorescent protein in early development and from mouse hemangioblasts expressing
Scl (truncated inactive human CD4 knock in) and Flk in an in vitro ES
cell differentiation assay. Gene expression profiles from these two cell
populations will be analyzed by direct sequencing of normalized cDNA clones.
This comparative gene expression analyses will improve our understanding of stem
cell biology at a molecular level.