INTRODUCTION:

 The Zebrafish is an excellent genetic and developmental system for studying embryonic patterning and specification, organogensis, and disease [1-3] .  As a part of the zebrafish genome project initiative, we have created this web page to help take advantage of the zebrafish genetic system.  The focus is on building a comprehensive radiation hybrid (RH) map for the zebrafish genome using available EST sequences and other genetic markers.  This map is helpful for isolating genes responsible for mutant phenotypes and for comparative genomics.

Radiation hybrid (RH) mapping is based on analysis of the marker distribution among a panel of host cell lines containing random genomic fragments of a target genome.  These fragments are either integrated into the host genome or present as microchromosomes in the host nucleus.  Radiation hybrid mapping has advantages over other mapping methods.  It does not require markers to be polymorphic between a mutant strain and a mapping strain.  It is faster and relatively inexpensive for constructing a high-resolution map.

Two RH panels are available for mapping zebrafish genes and markers.  The Goodfellow panel (also named RG panel, Goodfellow T51, Zebrafish/Hamster panel) was made by fusing irradiated zebrafish AB9 cells with Hamster Wg3H cells.  (AB9 is a fin cell line from the AB Fish.)  The Ekker panel (also named LN54 panel) was made by fusing irradiated AB9 cells with Mouse B78 cells.  The potential resolution of the Goodfellow panel and the Ekker panel are 350 KB and  500 KB, respectively.

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