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FORM B:  

Instructions on primer design: Primers designed from 3'UTR sequence has higher success rate for producing a map position.  Coding regions will work for PCR but may not be able to produce an accurate map location due to other family genes.  Try to avoid primers that span introns and highly conserved regions.  See the RH Mapping Protocols if you have scoring questions.  Our mapping effort primarily focuses on the Goodfellow panel.  If the Goodfellow panel fails to map your gene, we will try the Ekker panel for it.  If the Ekker panel is especially required for mapping your gene, please check the proper box. 

*Optional if unpublished.  Other fields are required.

**  If researcher is different from primary investigator (P.I.)

 Name of Researcher    

     E-Mail                               

      Affiliation                          

     Principal Investigator**

     Principal Investigator's E-Mail

     Phone                              

     Fax                                   

                        ( Country code is required if outside of U.S. or Canada)

     Actual Gene Name on Maps?  Yes     No

     Actual Gene name*       

     GenBank Accession No.*

     Forward primer name    

     Reverse primer name

     Forward primer sequence    F:  5'     3'                
                                                         F-Tm    oC

                                                         R:  5'    3'        
                                                         R-Tm    oC

 

   Optimal Annealing Temperature    oC

   Expected fragment size  

   Primer Tested ?     Yes      No                            

        Tested on Goodfellow panel only   

        On Ekker panel only                           

        On both panels                                    

  Have these primers been used for

       PCR reactions                  

       Sequencing                       

       Hybridization                     

        Others (please specify)   

        Is the Ekker panel mapping required?     Yes     No 

       

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